Pdf Evaluation By Flow Cytometry Of Mature Monocyte Subpopulations A startling idea suggests that monocyte subpopulations in blood are a “mirror of disrupted homeostasis and disease” (6). therefore, it is worth evaluating for the mature monocyte com partment. three subpopulations of mature monocytes (classical monocytes, noted hereafter as mo1; intermediate monocytes,. 12 the protocol for monocyte subset analysis is not uniform, as is the case for many flow cytometry assays. we acquired 300 000 total events with pb, 12 while others have used 50000 events in the.
Frontiers Evaluation By Flow Cytometry Of Mature Monocyte In addition, the monocyte subpopulations screening by mfc was performed in two aml npm1 patients in remission, showing a persistent monocytosis and dysplastic signs in bm evaluation, after consolidation therapy in one case, and after allogeneic stem cell transplantation for the second case. in both cases, an increased level of mo1 monocytes. The mfc test for the evaluation of the repartition of monocyte subsets, as previously described by selimoglu buet et al. showed a specificity of 97% in blood and 100% in marrow samples. additional information regarding the expression of intermediate mo2 monocytes percentage improved the specificity to 100% in blood samples allowing the. Citation: picot t, aanei cm, flandrin gresta p, noyel p, tondeur s, tavernier tardy e, guyotat d and campos catafal l (2018) evaluation by flow cytometry of mature monocyte subpopulations for the diagnosis and follow up of chronic myelomonocytic leukemia. front. oncol. 8:109. doi: 10.3389 fonc.2018.00109. received: 15 january 2018; accepted: 28. The mfc test for the evaluation of the repartition of monocyte subsets, as previously described by selimoglu buet et al. showed a specificity of 97% in blood and 100% in marrow samples. additional information regarding the expression of intermediate mo2 monocytes percentage improved the specificity to 100% in blood samples allowing the.
Frontiers Evaluation By Flow Cytometry Of Mature Monocyte Citation: picot t, aanei cm, flandrin gresta p, noyel p, tondeur s, tavernier tardy e, guyotat d and campos catafal l (2018) evaluation by flow cytometry of mature monocyte subpopulations for the diagnosis and follow up of chronic myelomonocytic leukemia. front. oncol. 8:109. doi: 10.3389 fonc.2018.00109. received: 15 january 2018; accepted: 28. The mfc test for the evaluation of the repartition of monocyte subsets, as previously described by selimoglu buet et al. showed a specificity of 97% in blood and 100% in marrow samples. additional information regarding the expression of intermediate mo2 monocytes percentage improved the specificity to 100% in blood samples allowing the. Control group. there was no association between monocyte subpopulations and major fibrotic manifestations. cd169 was shown to be more representative in dssc, being a promising marker for differentiating disease subtypes. keywords: systemic sclerosis, monocytes, monocyte subpopulations, flow cytometry, pathogenesis. Mrd evaluation using flow cytometry (fcm) has been proved to be a powerful tool [1,2]. routine fcm mrd assessment is usually limited to blast evaluation in non monocytic type of aml [ 1 ]. fcm assessment of monocytes and granulocytes in addition to blasts improved diagnostic accuracy in classification of aml with myelodysplasia related changes.
Comments are closed.